EC Number |
---|
3.7.1.2 | - |
3.7.1.2 | nickel-chelate affinity chromatography |
3.7.1.2 | recombinant enzyme 5.0fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and anion exchange chromatography |
3.7.1.2 | recombinant His-tagged enzyme |
3.7.1.2 | recombinant His-tagged enzyme, fusion protein is cleaved from the fusion tag by bovine thrombin |
3.7.1.2 | using a HiPrep 26/10 desalting column, a Super Q Toyopearl 650M column, a Resource Q column, a Bio-Scale CHT-10-I column, and a HiLoad 16/60 Superdex 200 pg column |