EC Number   |
|---|
    3.5.4.9 | - |
| 3.5.4.9 | by anion-exchange chromatography |
| 3.5.4.9 | His-Trap affinity column chromatography and Superdex-S200 gel filtration |
| 3.5.4.9 | Ni2+-charged HisTrap column chromatography and Superdex 200 gel filtration |
| 3.5.4.9 | recombinant DHCH1 protein is purified by metal-affinity chromatography |
| 3.5.4.9 | recombinant His-tagged enzyme by nickel affinity chromatography, cleavage of the His-tag by TEV protease, His affinity chromatography, and gel filtration, to over 95% purity |
| 3.5.4.9 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by Ni2+-chelate affinity chromatography |
| 3.5.4.9 | recombinant N-terminally 6-His-tagged MTHFDC from Escherichia coli strain BL21(DE3) by heat-treatment, nickel affinity chromatography, and gel filtration |
| 3.5.4.9 | recombinant protein expressed in Escherichia coli |
| 3.5.4.9 | recombinant protein expressed in Escherichia coli K38 |
