EC Number |
---|
3.5.1.9 | - |
3.5.1.9 | native enzyme from liver cytosol, recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography, to high purity, recombinant wild-type enzyme 90fold |
3.5.1.9 | near homogeneity, chromatography techniques |
3.5.1.9 | near homogeneity, chromatography techniques, isoelectric focusing |
3.5.1.9 | partial |
3.5.1.9 | recombinant enzyme from Escherichia coli by affinity chromatography, ion exchange chromatography, and gel filtration |
3.5.1.9 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration |
3.5.1.9 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, cleavage of the tag by tobacco etch virus, followed by another step of nickel affinity chromatography, gel filtration of the eluate |
3.5.1.9 | to homogeneity, chromatography steps |
3.5.1.9 | to homogeneity, chromatography steps, isoelectric focusing |