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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13- 37007, 37019, 37029
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13Ni-NTA column chromatography 710668
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13partial 37002, 37008
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13recombinant glutathione S-transferase fusion proteins of three segments of enzyme, comprising amino acids 1032-1964, 708-1964, and 104-1964. Segment 1032-1964 is catalytically inactive. Recombinant proteins are highly instable. 666834
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13recombinant N-terminally tagged wild-type and mutant active mature enzymes from Escherichia coli strain BL21(DE3) by nickel and amylose affinity chromatography, MBP-tag cleavage through TEV and the His-tag through thrombin, and gel filtration. Recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography an, tag cleavage through thrombin, and gel filtration 755335
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13type 1 enzyme 37003
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13type 2 enzyme 37002, 37003
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.13using Ni-NTA agorose binding 680136
Results 1 - 8 of 8