EC Number   |
|---|
   3.4.22.32 | - |
| 3.4.22.32 | 2 enzyme forms with pI of 9.45 (basic form) and pI 4.7 (acidic form) |
| 3.4.22.32 | 2 forms: SB1 and SB2 |
| 3.4.22.32 | 2 isozymes SBA/a and SBA/b |
| 3.4.22.32 | 6 enzyme forms: SBB 1-5 and SBA |
| 3.4.22.32 | affinity chromatography |
| 3.4.22.32 | bromelain A and B |
| 3.4.22.32 | bromelain from pineapple stem and skin are recovered by a PEG 4000/phosphate aqueous two-phase systems (ATPs) liquid-liquid extraction |
| 3.4.22.32 | bromelain is precipitated from the pineapple stem juice by 95% v/v ethanol and then freeze-dried or spray-dried to get the dry powder |
| 3.4.22.32 | carboxymethylcellulose 52 column chromatography, and Sephadex G-100 gel filtration |
