EC Number   |
|---|
   3.1.21.4 | - |
| 3.1.21.4 | ammonium sulfate fractionation, phosphocellulose P-11 column chromatography, heparin-Sepharose column chromatography, AH-Sepharose column chromatography, and Blue-Sepharose column chromatography |
| 3.1.21.4 | EcoRI and EcoRV |
| 3.1.21.4 | extremely fast and economical method of restriction endonucleases free from contaminating nuclease activity by a combination of affity partitioning and ion-exchange chromatography |
| 3.1.21.4 | heparin column chromatography, Source Q column chromatography, Source S column chromatography, Superdex 75 gel filtration, and ceramic HTP column chromatography |
| 3.1.21.4 | HiTrap heparin column chromatography |
| 3.1.21.4 | partial |
| 3.1.21.4 | phosphocellulose P-11 column chromatography, heparin Sepharose column chromatography, CM Sephadex C-50 gel filtration or Blue-agarose column chromatography |
| 3.1.21.4 | recombinant enzyme |
| 3.1.21.4 | wild-type and mutant enzymes |
