EC Number |
---|
2.7.4.27 | ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration |
2.7.4.27 | immobilized metal ion affinity chromatography (Ni2+) |
2.7.4.27 | partially purified |
2.7.4.27 | partially purified, ammonium sulfate precipitation, dye ligand chromatography (Blue-Sepharose, agarose-blue dextran), gel filtration |
2.7.4.27 | partially purified, ammonium sulfate precipitation, dye-ligand chromatography (Blue Sepharose), gel filtration, anion exchange chromatography |
2.7.4.27 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) RIL by nickel affinity chromatography |