EC Number   |
|---|
    2.7.1.148 | - |
| 2.7.1.148 | Hi-trap chelating columns are used for the purification of recombinant 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase |
| 2.7.1.148 | recombinant enzyme |
| 2.7.1.148 | recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography, tag cleavage through Prescission protease, followed by anion exchange chromatography, and gel filtration |
| 2.7.1.148 | recombinant His-tagged enzyme from strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography |
| 2.7.1.148 | recombinant His6-tagged enzyme by nickel affinity chromatography |
| 2.7.1.148 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, followed by anion exchange chromatography, and gel filtration |
| 2.7.1.148 | recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography, gel filtration, and ultrafiltration |
| 2.7.1.148 | The gene is preceded by a His6 tag to enable purification of the recombinant protein via metal-chelating affinity chromatography. The polyhistidine tag is removed by thrombin-mediated proteolysis, followed by purification with anion-exchange chromatography. Purity of the sample is assessed by SDS-PAGE and MALDI-TOF MS. |
