EC Number |
---|
2.5.1.65 | Ni-NTA column chromatography and Superdex-200 gel filtration |
2.5.1.65 | recombinant enzyme from Escherichia coli |
2.5.1.65 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography |
2.5.1.65 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration, followed by cleavage of the His-tag using thrombin and tag elimination through another sequence of nickel affinity chromatography and gel filtration |
2.5.1.65 | recombinant OASS |
2.5.1.65 | recombinant wild-type, selenomethinonine-labeled, and mutant R297A enzymes from Escherichia coli |
2.5.1.65 | using Ni-NTA chromatography |