EC Number   |
|---|
    2.3.1.24 | - |
| 2.3.1.24 | n-octyl beta-D-thioglucoside, DE-32, sphingosine-celite, Sepharose CL-6B |
| 2.3.1.24 | native enzyme 98.7fold from liver mitochondrionrich fraction by solubilization with n-octyl beta-D-thioglucoside, ion exchange chromatography, sphingosine affinity chromatography, and gel filtration |
| 2.3.1.24 | recombinant His-tagged LASS5 from MLE cells by nickel affinity chromatography and ultrafiltration |
| 2.3.1.24 | solubilization of membrane-bound enzyme from brain mitochondria by 2% of Trinton X-100, followed by ammonium sulfate fractionation and dialysis |
| 2.3.1.24 | subcellular fractionation of HeLa cells recombinantly expressing the CerS isozyme |
