EC Number |
---|
1.5.1.11 | - |
1.5.1.11 | 2 isoenzymes: A and B |
1.5.1.11 | ammonium sulfate precipitation, Ni2+-NTA column chromatography, and Sephadex G-100 gel filtration |
1.5.1.11 | crown-gall tumor-tissue induced by Agrobacterium tumefaciens |
1.5.1.11 | glutathione Sepharose column chromatography |
1.5.1.11 | His5-tagged OcDH and His6-tagged OcDH are purified by Ni-NTA chromatography, yielding almost 20 mg homogenous enzyme per litre of cell culture (purity greater than 98%). The purification of OcDH-tagless requires several chromatographic steps and yields 3-5 mg per litre of cell culture, with a purity of more than 98% |
1.5.1.11 | Ni-NTA affinity chromatography and Sephadex G-100 gel filtration |
1.5.1.11 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration |
1.5.1.11 | the biospecific chromatographic affinity purification is not applicable to the purification of the enzyme from Monodonta lineate |
1.5.1.11 | the biospecific chromatographic affinity purification is not applicable to the purification of the enzyme from Mytilus edulis |