EC Number   |
|---|
    1.3.8.1 | - |
| 1.3.8.1 | by chromatography and fractionation, to ca. 95% homogeneity |
| 1.3.8.1 | by gel filtration, to more than 95% purity |
| 1.3.8.1 | by successive fractionations of anion-exchange chromatography, ammonium sulfate precipitation and gel filtration, to more than 99% homogeneity |
| 1.3.8.1 | DEAE cellulose, gel filtration, partially purified |
| 1.3.8.1 | enzyme exhibits a bright green color from a strong absorption band at 710 nm due to tightly bound CoA persulfide |
| 1.3.8.1 | native enzyme from strain VR4 cell supernatant by ultracentrifugation, ammonium sulfate fractionation, dialysis, anion exchange chromatography, and ultrafiltration, the the enzyme is mixed with excess of solid dithionite under anaerobic conditions to give the yellow form, and further purified by desalting gel filtration under anaerobic conditions |
| 1.3.8.1 | preparation of apoprotein |
| 1.3.8.1 | preparation of CoA-persulfide free enzyme |
| 1.3.8.1 | pure enzyme has a characteristic green colour, CoA-persulfide may be the donor in a charge-transfer complex that is responsible for the green colour |
