EC Number   |
|---|
    1.3.1.98 | - |
| 1.3.1.98 | ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration |
| 1.3.1.98 | Ni-NTA agarose column chromatography |
| 1.3.1.98 | recombinant enzyme from strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and ion exchange chromatography to homogeneity |
| 1.3.1.98 | recombinant His-tagged enzyme from Escherichia coli K12S by nickel affinity chromatography |
| 1.3.1.98 | recombinant MurB from Escherichia coli strain BL21(DE3) |
| 1.3.1.98 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, followed by a second step of affinity chromatography to remove uncleaved protein and the His6-tagged TEV protease, further purification by preparation scale gel filtration, and ultrafiltration |
| 1.3.1.98 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity, native wild-type enzyme from Staphylococcus aureus 10fold by ammonium sulfate precipitation and two steps of anion exchange chromatography |
