EC Number |
---|
1.2.1.36 | - |
1.2.1.36 | after recombinant production in Escherichia coli BL-21DE3 |
1.2.1.36 | by gel filtration, more than 400fold with a yield of about 1%, to near homogeneity |
1.2.1.36 | Ni-affinity chromatography |
1.2.1.36 | Ni-NTA column chromatography |
1.2.1.36 | Ni2+-NTA chelating Sepharose column chromatography |
1.2.1.36 | nickel chelating resin column chromatography and Superdex 200 gel filtration |
1.2.1.36 | nickel-Sepharose column chromatography and Q-Sepharose column chromatography |
1.2.1.36 | on a Ni-NTA column |
1.2.1.36 | partially by isoelectric focusing |