Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Purification (Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 10 of 10
download as CSV
download all results as CSV
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50-
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50from culture extract to homogeneity, about 13fold
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50frozen pellet of cell culture is suspended in lysis buffer (50 mM Tris-HCl, pH 7.7, 025 mM phenylmethanesulfonyl fluoride, 1 microM pepstatin A, lysozyme), cells disrupted by sonication, centrifuged, supernatant purified by affinity chromatography, mixed with Ni-NTA agarose medium, washed, packed into a column, eluted, active fractions combined and stored in buffer (20 mM phosphate buffer, pH 7.0, 0.25 mM phenylmethanesulfonyl fluoride, 5% glycerol)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50partially
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50recombinant enzyme from Escherichia coli to homogeneity
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50recombinant enzyme from Pseudomonas aeruginosa to over 95% homogeneity by nickel affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50recombinant wild-type and mutant enzymes from Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.50recombinant wild-type and mutant from Escherichia coli as glutathione-S-transferase fusion proteins
Results 1 - 10 of 10
download as CSV
download all results as CSV