EC Number |
---|
1.11.1.18 | - |
1.11.1.18 | ammonium sulfate precipitation, Ni-NTA column chromatography, and DE52 gel filtration |
1.11.1.18 | BPO 1 |
1.11.1.18 | BPO 2 |
1.11.1.18 | isolation of three forms of CoVBPO: nVBPO which is isolated directly from the algal species, sVBPO which is produced in a soluble form by Escherichia coli and rVBPO which is expressed by Escherichia coli as inclusion bodies and subsequently refolded |
1.11.1.18 | Na3VO4 is added to the crude extract (1.12 mg protein/ml) followed by heat treatment at 70°C for 2 h, 30-55% (w/v) ammonium sulfate precipitation and DEAE-52column chromatography |
1.11.1.18 | native enzyme VBrPO(AnII) from algal powder by liquid-liquid-partitioning, dialysis, and ultracentrifugation |
1.11.1.18 | Ni2+-charged HiTrap chelating column chromatography |
1.11.1.18 | nonhaem-type bromoperoxidase BPO1a |
1.11.1.18 | nonhaem-type bromoperoxidase BPO1b |