EC Number   |
|---|
   1.11.1.12 | - |
| 1.11.1.12 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Sephadex G-50 gel filtration, and HiTrap SP column chromatography |
| 1.11.1.12 | by Ni2+-nitrilotriacetic acid Sepharose |
| 1.11.1.12 | by nickel affinity chromatography |
| 1.11.1.12 | from cytosol and mitochondria |
| 1.11.1.12 | large-scale, wild-type and mutant SeC46C from baculovirus/insect cell expression system and mutant SeC46C from expression in Escherichia coli as His-tagged fusion protein |
| 1.11.1.12 | on a Ni-NTA column, further purified by gel filtration |
| 1.11.1.12 | partially |
| 1.11.1.12 | partially from testis and to homogenity from sperm as recombinant enzyme expressed in Escherichia coli and as wild-type enzyme |
| 1.11.1.12 | Percoll step gradient and nickel affinity chromatography |
