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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.14recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Tuner(DE3)-pLysS by nickel affinity chromatography, dialysis under anaerobic conditions, and ultrafiltration 747765
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.14recombinant wild-type, selenomethionine-labeled, and mutant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel and heparin affinity chromatography 728401
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.14using a HisTrap HP 5-mL column 715668
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.14using Ni-NTA chromatography 714746
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