EC Number   |
|---|
   3.1.13.1 | purified on protein A-agarose and affinity GST-AtRrp4p inmobilized on nitrocellulose |
| 3.1.13.1 | recombinant |
| 3.1.13.1 | recombinant His- or FLAG-tagged wild-type non-acetylated and acetylated and mutants from Escherichia coli strain BL21(DE3) by affinity chromatography |
| 3.1.13.1 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 3.1.13.1 | recombinant RC-RNase 2 refolded from Escherichia coli strain BL21(DE3) inclusion bodies, further purification of the dialyzed enzyme by two different steps of cation exchange chromatography. The native enzyme is purified from oocytes by ultracentrifugation, extraction of the yolk granules from the pellet, followed by cation exchange chromatography |
| 3.1.13.1 | RNase R and RNase II constructs, full-length wild-type RNase R and RNase R mutant D278N |
| 3.1.13.1 | Rrp44-exosome (RE) architecture suggests an active site sequestration mechanism for strict control of 3' exoribonuclease activity in the RE complex |
| 3.1.13.1 | to apparent homogeneity, 270fold |
| 3.1.13.1 | wild-type and RNase II mutants purified by affinity chromatography |
