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5.4.99.64
synthesis
introduction of a 2-hydroxyisobutyric acid synthesis route in Escherichia coli by concomitantly expressing the RCM genes and phaA and phaB, encoding beta-ketothiolase and NADP-dependent (R)-3-hydroxybutyryl-CoA dehydrogenase. A concentration of 3-hydroxybutyric acid of up to 17.7 mM can be obtained within 8 days of feeding the recombinant strain with gluconic acid as the main carbon source
737504
5.4.99.64
synthesis
synthesis of 2-hydroxyisobutanoate in Cupriavidus necator H16 expressing mutase genes HcmA and HcmB. Due to the enantiospecificity of the mutase, fructose is a weaker substrate for 2-hydroxyisobutanoate synthesis than butanoate. Production rates achieved with the poly-3-hydroxybutanoate-negative strain H16 PHB?4 on butanoate are higher than on fructose. Using the wild-type does not significantly improve the production rates, the latter shows a 34fold and a 5fold lower 2-hydroxyisobutanoate synthesis rate compared to H16 PHB?4 on fructose and butanoate, respectively. Both strains show concomitant excretion of undesired side products, such as pyruvate and 3-hydroxybutanoate
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737532
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