EC Number   |
Application |
Reference |
|---|
   3.1.21.4 | analysis |
genetic analysis of 24 Hungarian canine parvovirus strains collected from 2004 to 2008 revealed that all of them are type 2a strains. Due to a seemingly constant point mutation present in most of the Hungarian canine parvovirus 2a strains, a previously described MboII-based rapid identification of CPV2c strains unfortunately cannot be reliably used any more |
710678 |
| 3.1.21.4 | analysis |
method for following the digestion of DNA by restriction endonucleases in real time without the use of any extrinsic dyes or labels via linear dichroism spectroscopy |
683156 |
| 3.1.21.4 | analysis |
the enzyme can be used in DNA-based diagnostic applications |
134258 |
| 3.1.21.4 | analysis |
type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA |
-, 714119 |
| 3.1.21.4 | biotechnology |
evolvement of mutant enzymes with altered DNA cleavage specificities by application of an in vivo positive and negative selection system that applies evolutionary pressure either to favor the cleavage of a desired target sequence or to disfavor the cleavage of a nontarget sequence |
683588 |
| 3.1.21.4 | biotechnology |
generation of cleavage specificities of restriction endonucleases by swapping putative target recognition domains between the type IIB enzymes AloI, PpiI from Pseudomonas putida, and TstI from Thermus scotoductus. Individual target recognition domains recognize distinct parts of the bipartite DNA targets of these enzymes and are interchangeable. Engineering of a functional type IIB restriction endonuclease having previously undescribed DNA specificity and application in generation of type II enzymes with predetermined specificity |
-, 684004 |
| 3.1.21.4 | molecular biology |
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for future studies of large sets of restriction endonucleases and their activity |
751913 |
| 3.1.21.4 | molecular biology |
a straightforward, general and automatable model system for studying the activity of restriction endonucleases by using massively parallel sequencing is described, which should be highly applicable for the future studies of large sets of restriction endonucleases and their activity |
-, 751913 |
| 3.1.21.4 | molecular biology |
protein tagging with a wide variety of epitopes and/or fusion partners is used routinely to dissect protein function molecularly. Frequently the required DNA subcloning is inefficient, especially in cases where multiple constructs are desired for a given protein with unique tags. The generated clones have unwanted junction sequences introduced. To add versatile tags into the extracellular domain of the transmembrane protein THSD1, a protein tagging technique is developed that utilizes non-classical type IIS restriction enzymes that recognize non-palindromic DNA sequences and cleave outside of their recognition sites. The method is highly efficient and can precisely fuse any tag into any position of a protein in a scarless manner. IT is cost-efficient and adaptable because it uses commercially available type IIS restriction enzymes and is compatible with the traditional cloning system used by many labs |
749816 |
| 3.1.21.4 | molecular biology |
site-directed mutagenesis methods are very important in modern molecular biology, biochemistry, and protein engineering. A site-directed mutagenesis method that can be used for multiple mutation generation using type IIs restriction enzymes. This approach is faster and more convenient than the overlap polymerase chain reaction method due to its having fewer reaction steps and being cheaper than, but as convenient as, enzymatic assembly |
749557 |
