EC Number   |
Application |
Reference |
|---|
   2.7.7.7 | analysis |
the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications |
726060 |
| 2.7.7.7 | biotechnology |
DNA polymerase plays prominent roles in numerous biotechnologies. The use of diphosphate substrates has the potential to make practical the incorporation of expensive analogs, such as isotopically labeled or chemically modified nucleotides, eliminating the need for challenging triphosphate syntheses. This feature of DNA polymerases may also provide a method for detecting nucleotides used in high-throughput DNA sequencing |
-, 762278 |
| 2.7.7.7 | biotechnology |
short-patch compartmentalized self-replication will be a powerful strategy for the generation of polymerases with altered substrate specificity for applications in nano- and biotechnology and in the enzymatic synthesis of antisense and RNAi probes |
675398 |
| 2.7.7.7 | biotechnology |
thermostable polymerase used in PCR |
-, 643614, 643617, 643634, 643644, 643654, 643671, 643675, 643677, 643678 |
| 2.7.7.7 | diagnostics |
a mutated thermostable DNA polymerase, Taq M1, from Thermus aquaticus, that exhibits an increased reverse transcriptase activity, is therefore designated for one-step PCR pathogen detection using established real-time detection methods |
721895 |
| 2.7.7.7 | diagnostics |
rational design approach to creating modulated proofreading DNA polymerases which can be utilized in a highly sensitive long RT/PCR amenable to the clinical diagnostic setting |
-, 672087 |
| 2.7.7.7 | diagnostics |
the enzyme is useful in DNA amplification and PCR-based applications, particularly in clinical diagnoses using uracil-DNA glycosylase |
726710 |
| 2.7.7.7 | drug development |
DNA polymerases are promising drug targets for the treatment of cancer |
721129 |
| 2.7.7.7 | medicine |
adenovirus DNA polymerase is a target for immunotherapy of adenovirus disease |
704919 |
| 2.7.7.7 | medicine |
clinically, POLG mutations can present from early neonatal life to late middle age, with a spectrum of phenotypes that includes common neurological disorders such as migraine, epilepsy and Parkinsonism |
673942 |
