EC Number   |
Application |
Reference |
|---|
  2.1.1.111 | synthesis |
expression of anthranilate N-methyltransferase, anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and anthranilate coenzyme A ligase from Pseudomonas aeruginosa in Escherichia coli mutants mutants (metJ, trpD, tyrR), which provide more anthranilate and/or S-adenosyl methionine, to synthesize N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate. Approximately, 185.3 microM N-methylanthranilate and 95.2 microM methyl N-methylanthranilate are synthesized |
757422 |
| 2.1.1.111 | synthesis |
production of 4-hydroxycoumarin, 2,4-dihydroxyquinoline, and 4-hydroxy-1-methyl-2(1H)-quinolone in Escherichia coli using genes for the synthesis of salicylic acid from chorismate to supply the substrate for 4-hydroxycoumarin and the gene encoding N-methyltransferase for the synthesis of N-methylanthranilate from anthranilate. 255.4 mg/l 4-hydroxycoumarin, 753.7 mg/l dihydroxyquinoline, and 17.5 mg/l 4-hydroxy-1-methyl-2(1H)-quinolone are synthesized |
757053 |
| 2.1.1.111 | synthesis |
three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate are synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa are cloned and Escherichia coli strains harboring these genes were used to synthesize the three desired compounds. Escherichia coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, are used to increase the production of the synthesized compounds. 0.1853 mM N-methylanthranilate and 0.0952 mM methyl N-methylanthranilate are synthesized |
757422 |
