EC Number |
Application |
Reference |
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1.14.13.163 | synthesis |
development of an efficient process to transform HSP into 2,5-dihydroxypyridine (2,5-DHP) with heterologously expressed HSP hydroxylase and NADH-regenerating system, because 2,5-DHP, the product of the reaction catalyzed by HSP hydroxylase, is a valuable precursor for the chemical synthesis of 5-aminolevulinic acid, which is applied as a plant growth hormone, a herbicide and in cancer therapy |
-, 746248 |
1.14.13.163 | synthesis |
enzyme HSPHZZ can be used for the enzymatic production of 2,5-dihydroxypyridine in biotechnology applications. 85.3 mg/l 2,5-dihydroxypyridine is produced in 40 min with a conversion of 74.9% at 30°C, pH 8.5, 1.0 mM substrate concentration, and 0.001 mM enzyme concentration |
744640 |
1.14.13.163 | synthesis |
production of 2,5-dihydroxypyridine from 6-hydroxy-3-succinoylpyridine in the presence of NADH and FAD using nicotine hydroxylase covalently immobilized on Immobead 150. At a protein loading of 15 mg/g, ImmHSPHZZ converts 93.6% of 6-hydroxy-3-succinoylpyridine to 2,5-dihydroxypyridine in 6 h. The optimal concentrations of ImmHSPHZZ and substrate are 30 mg/l and 0.75 mM, respectively. Under optimal conditions, 94.5 mg/l of 2,5-dihydroxypyridine is produced after 30 min with 85.4% conversion |
764424 |