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EC Number Application Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.26synthesis asymmetric dihydroxylation of non-natural cinnamonitrile to trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile by chlorobenzene dioxygenase in recombinant Escherichia coli JM101 (pTEZ30). Recombinant hosts with a strong expression system, such as Escherichia coli JM101 (pTEZ30), can be used to produce dioxygenases efficiently to performcis-dihydroxylations up to technical scales. The cells should be maintained in ametabolically active state duringthe biotransformation in order to increase and maintain the volumetric productivity for long-term reactions 746688
Display the word mapDisplay the reaction diagram Show all sequences 1.14.12.26synthesis the enzyme is cloned under the strict control of the Palk promoter of Pseudomonas putida GPo1 and expressed in Escherichia coli JM101 cells carrying the resulting plasmid pTEZ30. Cells are used for regio- and stereoselective dihydroxylation of benzonitrile, ortho-, meta- and para-substituted benzonitriles, as well as cinnamonitrile and benzyl cyanide. cis-Dihydroxylations are at the 1,2 positions and the products have 42.9 to 97.1% enantiomeric excess 746689
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