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EC Number Organic Solvent Commentary Reference
Show all pathways known for 5.3.3.1Display the word mapDisplay the reaction diagram Show all sequences 5.3.3.1urea the recovery of the activity by refolding is not diminished even after a prolonged time, 24 h of exposure to 7 M urea. Reversibility of the folding is assessed by a 100fold dilution of the denatured protein and subsequent determination of enzyme activity. The refolding kinetics as monitored by fluorescence intensity can be described as a fast first-order process followed by a second-order and a subsequent slow first-order processes, there may be a monomeric folding intermediate 649932
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