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EC Number
Natural Substrates
Commentary (Nat. Sub.)
5.4.99.23
23S rRNA uridine1911/uridine1915/uridine1917
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5.4.99.23
23S rRNA uridine1911/uridine1915/uridine1917
pseudouridine synthase RluD converts uridines at positions 1911, 1915, and 1917 of 23S rRNA to pseudouridines. These nucleotides are located in the functionally important helix-loop 69 of 23S rRNA. RluD is the only pseudouridine synthase that is required for normal growth in Escherichia coli
5.4.99.23
23S rRNA uridine1911/uridine1915/uridine1917
RluD is far more efficient in modifying uridine residues in helix 69 in structured 50S subunits, compared to free or synthetic 23S rRNA. It is suggested that pseudouridine modifications in helix 69 are made late in the assembly of 23S rRNA into mature 50S subunits
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