EC Number   |
Natural Substrates |
|---|
   3.1.26.4 | RNA-DNA hybrid + H2O |
RNases H3 recognizes the 2'-OH groups of the RNA strand and detects the DNA strand by binding a phosphate group and inducing B-form conformation |
| 3.1.26.4 | RNA-DNA hybrid + H2O |
the enzyme specifically cleaves the RNA strand of RNA/DNA hybrids |
| 3.1.26.4 | RNA-DNA*DNA hybrid + H2O |
enzyme removes RNA primers from lagging strand fragments during DNA replication, 5'-to 3'-exonuclease activity, degradation of the RNA portion of the duplex |
| 3.1.26.4 | RNA-DNA*DNA hybrid + H2O |
RNA primer recognition and removal during DNA replication |
| 3.1.26.4 | RNA-DNA/DNA hybrid + H2O |
PabRNase HII acts as a specific endonuclease on RNA-DNA/DNA duplexes. Specific cleavage, one nucleotide upstream of the RNA-DNA junction, occurs on a substrate in which RNA initiators is fully annealed to the cDNA template. Additionally, PabRNase HII cleaves a single ribonucleotide embedded in a double-stranded DNA |
| 3.1.26.4 | ssDNA-dsDNA + H2O |
5'-to 3'-exonuclease activity, exonuclease activity removing short oligonucleotides of 3-30 nucleotides from adjacent DNA |
