EC Number   |
Metals/Ions |
Reference |
|---|
    6.1.1.1 | K+ |
0.15 M activates 12fold |
39 |
| 6.1.1.1 | K+ |
absolute requirement, Km: 10 mM |
47 |
| 6.1.1.1 | K+ |
activates the wild-type enzyme and mutants S225A and K231A, no interaction with the KMSSS motif |
652281 |
| 6.1.1.1 | K+ |
activation up to a concentration of 200 mM, sharp decrease above |
23 |
| 6.1.1.1 | K+ |
dependent on, required for catalysis of the tyrosine activation step, 260fold increase of the froward reaction constant |
652238 |
| 6.1.1.1 | Mg2+ |
- |
653754, 675706, 676126, 676225, 676862, 677165, 694895, 704609 |
| 6.1.1.1 | Mg2+ |
10 mM, can be replaced by Mn2+ with equal efficiency and by Ca2+, Co2+, Sr2+, Ba2+, spermine with reduced efficiency |
39 |
| 6.1.1.1 | Mg2+ |
Km: 1 mM |
47 |
| 6.1.1.1 | Mg2+ |
no requirement if spermine is present |
38 |
| 6.1.1.1 | Mg2+ |
optimum at 6 mM |
23 |
