EC Number   |
Metals/Ions |
Reference |
|---|
   3.1.13.1 | Mn2+ |
increases activity |
656668 |
| 3.1.13.1 | Mn2+ |
necessary for activity |
134049, 134054 |
| 3.1.13.1 | Mn2+ |
optimum: 0.3 mM, stimulation about two-thirds the rate with Mg2+ |
134054 |
| 3.1.13.1 | Na+ |
increases activity against T4 mRNA, can substitute for K+ in activation against T4 mRNA and RNA |
134052 |
| 3.1.13.1 | NaCl |
enzyme is active in NaCl, but the cleavage efficiency is 10times lower when compared to its activity in KCl |
730689 |
| 3.1.13.1 | NH4+ |
activating |
134050, 134052, 134054 |
| 3.1.13.1 | NH4+ |
can substitute for K+ to a small extend |
134050, 134052, 134055 |
| 3.1.13.1 | NH4+ |
stimulates at pH 9.5 about 100%, optimum: 50 mM |
134054 |
| 3.1.13.1 | Zn2+ |
induces a folding reaction resulting in protein preparations with 50-60% alpha-helical content and 10-20% beta-sheet structure, critical concentration for refolding bigger or equal to 0.1 mM |
666317 |
