EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.2.4.1 | 0.00158 |
- |
thiamine diphosphate |
wild-type enzyme |
348995 |
| 1.2.4.1 | 0.0021 |
- |
pyruvate |
mutant S283C, pH 7.0, 25°C |
655474 |
| 1.2.4.1 | 0.0027 |
- |
thiamine diphosphate |
mutant Y281S/R282S, pH 7.0, 25°C |
655474 |
| 1.2.4.1 | 0.0029 |
- |
thiamine diphosphate |
mutant Y281A/R282A/S283A, pH 7.0, 25°C |
655474 |
| 1.2.4.1 | 0.003 |
- |
thiamine diphosphate |
mutant Y281A/R282A, pH 7.0, 25°C |
655474 |
| 1.2.4.1 | 0.0052 |
- |
thiamine diphosphate |
mutant R282A, pH 7.0, 25°C |
655474 |
| 1.2.4.1 | 0.0055 |
- |
thiamine diphosphate |
mutant Y281A, pH 7.0, 25°C |
655474 |
| 1.2.4.1 | 0.00665 |
- |
thiamine diphosphate |
Y177A mutant enzyme |
348995 |
| 1.2.4.1 | 0.0088 |
- |
pyruvate |
mutant R282A, pH 7.0, 25°C |
655474 |
| 1.2.4.1 | 0.01 |
- |
pyruvate |
pH 7.6, 30°C, presence of 0.3 M KCl |
672086 |
