EC Number |
General Stability |
Reference |
---|
3.5.4.6 | an apparent 10000 Da reduction in molecular mass of the native 85000 Da enzyme subunit with its conversion to a 75000 Da core is observed on storage of AMPD |
685353 |
3.5.4.6 | ATP, ADP, GTP and alkali metal ions protect against heat inactivation |
209674 |
3.5.4.6 | EDTA stabilizes the purified enzyme during storage |
654859 |
3.5.4.6 | enzyme is associated to the histidine-proline-rich-glycoprotein HPRG via its catalytic subunit in a protein-protein complex, which is critical for the enzyme stability |
654859 |
3.5.4.6 | freezing, unstable |
209679 |
3.5.4.6 | heating in the presence of low KCl (150 mM) results in a rapid loss in activity with only 25% of initial activity being left after 30 min, about half of the initial activity remains after heating for 30 min in 25 mM histidine solution, solution, incubation in imidazole or phosphate solutions results in greater stability over 30 min of heating with AMPD activity decreasing by about 20% |
686221 |
3.5.4.6 | more |
654859 |
3.5.4.6 | phosphate stabilizes the tetrameric structure of the enzyme |
654859 |
3.5.4.6 | repeated freezing and thawing, stable |
209672 |
3.5.4.6 | sulfhydryl reagents and monovalent cations in high concentrations stabilize |
209679 |