Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Le Saux, O.; Gonzales, T.; Robert-Baudouy, J.
    Mutational analysis of the active site of Pseudomonas fluorescens pyrrolidone carboxyl peptidase (1996), J. Bacteriol., 178, 3308-3313.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.3 expression in Escherichia coli, wild-type and mutant enzymes Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.19.3 C144A inactive enzyme Pseudomonas fluorescens
3.4.19.3 D89A no activity Pseudomonas fluorescens
3.4.19.3 D89N 35% of activity of the wild-type enzyme Pseudomonas fluorescens
3.4.19.3 E10Q enzyme displays catalytic properties and sensitivities to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide similar to those of wild-type enzyme Pseudomonas fluorescens
3.4.19.3 E22Q enzyme displays catalytic properties and sensitivities to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide similar to wild-type enzyme Pseudomonas fluorescens
3.4.19.3 H166S inactive enzyme Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.3 additional information
-
 additional information
-
 Pseudomonas fluorescens
3.4.19.3 0.05
-
 Pyroglutamyl-beta-naphthylamide mutant enzyme D89N Pseudomonas fluorescens
3.4.19.3 0.21
-
 Pyroglutamyl-beta-naphthylamide wild-type enzyme Pseudomonas fluorescens
3.4.19.3 0.22
-
 Pyroglutamyl-beta-naphthylamide mutant enzyme E22Q Pseudomonas fluorescens
3.4.19.3 0.24
-
 Pyroglutamyl-beta-naphthylamide mutant enzyme E10Q Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 pyroglutamyl-Ala + H2O
-
 Pseudomonas fluorescens pyroglutamate + Ala
-
 ?
3.4.19.3 pyroglutamyl-beta-naphthylamide + H2O
-
 Pseudomonas fluorescens pyroglutamate + beta-naphthylamine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.19.3 7.4
-
 Pyroglutamyl-beta-naphthylamide mutant enzyme D89N Pseudomonas fluorescens
3.4.19.3 28
-
 Pyroglutamyl-beta-naphthylamide mutant enzyme E10Q Pseudomonas fluorescens
3.4.19.3 31.4
-
 Pyroglutamyl-beta-naphthylamide wild-type enzyme Pseudomonas fluorescens
3.4.19.3 35.1
-
 Pyroglutamyl-beta-naphthylamide mutant enzyme E22Q Pseudomonas fluorescens