Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marseno, D.W.; Hori, K.; Miyazawa, K.
    Purification and properties of cytosol 5'-nucleotidase from black rockfish (Sebastes inermis) muscle (1993), J. Agric. Food Chem., 41, 1208-1212.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 1,10-phenanthroline inhibition of cytosolic and membrane-bound enzyme Sebastes inermis
3.1.3.5 benzoic acid inhibition of cytosolic enzyme Sebastes inermis
3.1.3.5 L-ascorbic acid inhibition of cytosolic enzyme Sebastes inermis
3.1.3.5 p-chloromercuribenzoate inhibition of cytosolic and membrane-bound enzyme Sebastes inermis
3.1.3.5 phenylmethylsulfonyl fluoride inhibition of cytosolic enzyme Sebastes inermis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.0307
-
 5'-AMP cytosolic enzyme Sebastes inermis
3.1.3.5 0.0622
-
 5'-IMP cytosolic enzyme Sebastes inermis
3.1.3.5 0.131
-
 5'-UMP cytosolic enzyme Sebastes inermis
3.1.3.5 0.215
-
 5'-GMP cytosolic enzyme Sebastes inermis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cytoplasm
-
 Sebastes inermis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Co2+ activates cytosolic and membrane-bound enzyme Sebastes inermis
3.1.3.5 Mn2+ activates cytosolic and membrane-bound enzyme Sebastes inermis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 94000
-
 1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE Sebastes inermis
3.1.3.5 148000
-
 1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE Sebastes inermis
3.1.3.5 250000
-
 cytosolic enzyme, gel filtration Sebastes inermis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Sebastes inermis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 muscle
-
 Sebastes inermis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 28
-
 cytosolic enzyme Sebastes inermis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Sebastes inermis adenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Sebastes inermis guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Sebastes inermis inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Sebastes inermis uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 dimer 1 * 148000 + 1 * 94000, cytsolic enzyme, SDS-PAGE Sebastes inermis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.5 40
-
 cytosolic enzyme stable for 30 min Sebastes inermis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.5 7 9 cytosolic enzyme, 4°C, 16 h stable Sebastes inermis