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Literature summary extracted from

  • Yang, W.J.; Matsuda, Y.; Sano, S.I.; Masutani, H.; Nakagawa, H.
    Purification and characterization of phytase from rat intestinal mucosa (1991), Biochim. Biophys. Acta, 1075, 75-82.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 EDTA
-
 Rattus norvegicus
3.1.3.26 Zn2+ moderately inhibited by up to 2 mM ZnCl2, strongly inhibited at higher concentrations Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.12
-
 myo-inositol hexakisphosphate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Mg2+ required Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 70000
-
 x * 70000 + x * 90000, SDS-PAGE Rattus norvegicus
3.1.3.26 90000
-
 x * 70000 + x * 90000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Rattus norvegicus
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.26 side-chain modification the phytase/alkaline phosphatase of the intestine contains only the complex type of N-linked oligosaccharide, the 90000 Da subunit contains O-linked oligosaccharide chains with an apparent molecular mass of 11000 Da. The 70000 Da subunit does not contain oligosaccharide chains Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 intestinal mucosa
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 ADP + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.26 AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?
3.1.3.26 ATP + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.26 glucose 1-phosphate + H2O
-
 Rattus norvegicus glucose + phosphate
-
 ?
3.1.3.26 Glucose 6-phosphate + H2O
-
 Rattus norvegicus Glucose + phosphate
-
 ?
3.1.3.26 inositol 1,4-diphosphate + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.26 inositol pentakisphosphate + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.26 inositol-1-phosphate + H2O
-
 Rattus norvegicus inositol + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Rattus norvegicus myo-inositol pentakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 ? x * 70000 + x * 90000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 7.5 8 hydrolysis of myo-inositol hexakisphosphate Rattus norvegicus
3.1.3.26 10 10.4 hydrolysis of p-nitrophenyl phosphate Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.26 6 9 about 40% of maximal activity at pH 6.0 and 9.0, hydrolysis of myo-inositol hexakisphosphate Rattus norvegicus
3.1.3.26 9 11 pH 9.0: about 50% of maximal activity, pH 11.0: about 25% of maximal activity, hydrolysis of p-nitrophenyl phosphate Rattus norvegicus