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Literature summary extracted from

  • Laboure, A.M.; Gagnon, J.; Lescure, A.M.
    Purification and characterization of a phytase (myo-inositol-hexakisphosphate phosphohydrolase) accumulated in maize (Zea mays) seedlings during germination (1993), Biochem. J., 295, 413-419.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 Fe2+
-
 Zea mays
3.1.3.26 Zn2+
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.117
-
 myo-inositol hexakisphosphate
-
 Zea mays
3.1.3.26 0.133
-
 myo-inositol pentakisphosphate
-
 Zea mays
3.1.3.26 0.181
-
 diphosphate
-
 Zea mays
3.1.3.26 0.227
-
 ATP
-
 Zea mays
3.1.3.26 0.25
-
 myo-inositol trikisphosphate
-
 Zea mays
3.1.3.26 0.555
-
 p-nitrophenyl phosphate
-
 Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Ca2+ 2 mM, stimulates 35% over the control Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 38000
-
 2 * 38000, SDS-PAGE Zea mays
3.1.3.26 76000
-
 non-denaturing PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.26 seedling during germination Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 2.288
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 ATP + H2O
-
 Zea mays ?
-
 ?
3.1.3.26 diphosphate + H2O
-
 Zea mays 2 phosphate
-
 ?
3.1.3.26 inositol pentakisphosphate + H2O myo-inositol pentakisphosphate Zea mays ?
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Zea mays myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol trikisphosphate + H2O
-
 Zea mays ?
-
 ?
3.1.3.26 p-nitrophenyl phosphate + H2O
-
 Zea mays p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 dimer 2 * 38000, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 55
-
-
 Zea mays

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.26 45 60 45°C: about 60% of maximal activity Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 55
-
 10 min, stable Zea mays
3.1.3.26 65
-
 10 min, 70% loss of activity Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 4.8
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.26 4 5.7 pH 4.0: about 65% of maximal activity, pH 5.7: about 75% of maximal activity Zea mays