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Literature summary extracted from

  • Schlömann, M; Ngai, K.L.; Ornston, L.N.; Knackmuss, H.J.
    Dienelactone hydrolase from Pseudomonas cepacia (1993), J. Bacteriol., 175, 2994-3001.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.45 EDTA no inactivation Burkholderia cepacia
3.1.1.45 additional information no inhibition by trans-4-carboxymethylenebut-2-en-4-olide, (+)-muconolactone, (+)-4-fluoromuconolactone Burkholderia cepacia
3.1.1.45 p-chloromercuribenzoate not inhibitory Burkholderia cepacia
3.1.1.45 succinate reversible inhibitory effect Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.45 0.59
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.45 39000
-
 gel filtration Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.45 additional information Burkholderia cepacia natural function is not in the degradation of haloaromatic compounds, the normal substrate might well be one of the more complex natural compounds with a dienelactone structure, or it could also be an intermediate of some other metabolic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.45 Burkholderia cepacia
-
 type II DLH: cis-dienelactone hydrolase
-
3.1.1.45 Burkholderia cepacia
-
 Pseudomonas cepacia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.45
-
 Burkholderia cepacia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.45 217
-
-
 Burkholderia cepacia

Storage Stability

EC Number Storage Stability Organism
3.1.1.45 -20°C, 5 months, specific activity decreases from 217 to 65 U/mg Burkholderia cepacia
3.1.1.45 20°C, sodium succinate, pH 5, perfectly stable during incubation overnight Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.45 cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Burkholderia cepacia 4-oxohex-2-enedioate
-
 ?
3.1.1.45 additional information muconolactone is not hydrolyzed to a significant extent Burkholderia cepacia ?
-
 ?
3.1.1.45 additional information natural function is not in the degradation of haloaromatic compounds, the normal substrate might well be one of the more complex natural compounds with a dienelactone structure, or it could also be an intermediate of some other metabolic pathway Burkholderia cepacia ?
-
 ?
3.1.1.45 trans-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Burkholderia cepacia 4-oxohex-2-enedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.45 monomer 1* 32400, SDS-PAGE Burkholderia cepacia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.45 817
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.45 5.5
-
-
 Burkholderia cepacia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.45 4.5 8.1
-
 Burkholderia cepacia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.45 5
-
 most stable around, decreasing faster towards lower than towards higher pH values Burkholderia cepacia