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Literature summary extracted from

  • Hatamoto, O.; Watarai, T.; Kikuchi, M.; Mizusawa, K.; Sekine, H.
    Cloning and sequencing of the gene encoding tannase and a structural study of the tannase subunit from Aspergillus oryzae (1996), Gene, 175, 215-221.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.20 cloned in Escherichia coli JM 109 and expressed in Aspergillus oryzae strain AO1, a tannase low-producing and nitrate reductase-deficient strain Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 30000
-
 x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE Aspergillus oryzae
3.1.1.20 33000
-
 x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE Aspergillus oryzae
3.1.1.20 290000
-
 strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, sedimentation equilibrium Aspergillus oryzae
3.1.1.20 310000
-
 strain AO1, glycoprotein containing 22.7% sugars, a hetero-octamer with four pairs of two subunits, gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Aspergillus oryzae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 purified from the strain AO1 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Aspergillus oryzae gallic acid + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus oryzae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 oligomer x * 30000 + x * 33000, four pairs of two subunits form a hetero-oligomer of a about 300000 Da native tannase, SDS-PAGE Aspergillus oryzae