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Literature summary extracted from

  • Che Omar, I.; Hayashi, M.; Nagai, S.
    Purification and some properties of a thermostable lipase from Humicola lanuginosa No. 3 (1987), Agric. Biol. Chem., 51, 37-45.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Co2+
-
 Thermomyces lanuginosus
3.1.1.3 Cu2+
-
 Thermomyces lanuginosus
3.1.1.3 EDTA 1 mM, 11% inhibition Thermomyces lanuginosus
3.1.1.3 Hg2+
-
 Thermomyces lanuginosus
3.1.1.3 Mg2+ 1 mM, 17% inhibition Thermomyces lanuginosus
3.1.1.3 Ni2+
-
 Thermomyces lanuginosus
3.1.1.3 Sn2+
-
 Thermomyces lanuginosus
3.1.1.3 Zn2+ 1 mM, 17% inhibition Thermomyces lanuginosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 additional information
-
 additional information Km-value: 14.2 mg/ml for trilaurin Thermomyces lanuginosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ enhances activity Thermomyces lanuginosus
3.1.1.3 K+ enhances activity Thermomyces lanuginosus
3.1.1.3 Li+ enhances activity Thermomyces lanuginosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 39000
-
 gel filtration Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Thermomyces lanuginosus
-
 No. 3
-
3.1.1.3 Thermomyces lanuginosus No. 3
-
 No. 3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3
-
 Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,2-dioleoylglycerol + H2O
-
 Thermomyces lanuginosus oleic acid + ?
-
 ?
3.1.1.3 1,2-dioleoylglycerol + H2O
-
 Thermomyces lanuginosus No. 3 oleic acid + ?
-
 ?
3.1.1.3 methyl linoleate + H2O
-
 Thermomyces lanuginosus methanol + linolic acid
-
 ?
3.1.1.3 methyl linolenate + H2O
-
 Thermomyces lanuginosus methanol + linoleic acid
-
 ?
3.1.1.3 methyl oleate + H2O
-
 Thermomyces lanuginosus methanol + oleic acid
-
 ?
3.1.1.3 methyl oleate + H2O
-
 Thermomyces lanuginosus No. 3 methanol + oleic acid
-
 ?
3.1.1.3 additional information 1,3-positional specific lipase Thermomyces lanuginosus ?
-
 ?
3.1.1.3 additional information 1,3-positional specific lipase Thermomyces lanuginosus No. 3 ?
-
 ?
3.1.1.3 polyethylene sorbitan monooleate + H2O i.e. Tween 80 Thermomyces lanuginosus ?
-
 ?
3.1.1.3 Span 85 + H2O
-
 Thermomyces lanuginosus ?
-
 ?
3.1.1.3 trilaurin + H2O
-
 Thermomyces lanuginosus lauric acid + ?
-
 ?
3.1.1.3 trilaurin + H2O
-
 Thermomyces lanuginosus No. 3 lauric acid + ?
-
 ?
3.1.1.3 trioleoylglycerol + H2O
-
 Thermomyces lanuginosus oleic acid + ?
-
 ?
3.1.1.3 trioleoylglycerol + H2O
-
 Thermomyces lanuginosus No. 3 oleic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 39000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 45
-
-
 Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 45
-
 24 h, stable Thermomyces lanuginosus
3.1.1.3 60
-
 20 h, stable Thermomyces lanuginosus
3.1.1.3 65
-
 60 min, stable Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
-
 Thermomyces lanuginosus