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Literature summary extracted from

  • Tobias, A.M.; Toska, D.; Lange, K.; Eck, T.; Bhat, R.; Janson, C.A.; Rotella, D.P.; Gubler, U.; Goodey, N.M.
    Expression, purification, and inhibition profile of dihydrofolate reductase from the filarial nematode Wuchereria bancrofti (2018), PLoS ONE, 13, e0197173 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expression in Escherichia coli including 13 amino acid loop Brugia malayi
1.5.1.3 expression in Escherichia coli including 13 amino acid loop Wuchereria bancrofti

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 (-)-epicatechin
-
 Brugia malayi
1.5.1.3 (-)-epicatechin
-
 Wuchereria bancrofti
1.5.1.3 (-)-epicatechin gallate
-
 Brugia malayi
1.5.1.3 (-)-epicatechin gallate
-
 Wuchereria bancrofti
1.5.1.3 aminopterin
-
 Brugia malayi
1.5.1.3 aminopterin
-
 Wuchereria bancrofti
1.5.1.3 methotrexate
-
 Brugia malayi
1.5.1.3 methotrexate
-
 Wuchereria bancrofti
1.5.1.3 pyrimethamine
-
 Brugia malayi
1.5.1.3 pyrimethamine
-
 Wuchereria bancrofti
1.5.1.3 raltitrexed
-
 Brugia malayi
1.5.1.3 raltitrexed
-
 Wuchereria bancrofti
1.5.1.3 trimethoprim
-
 Brugia malayi
1.5.1.3 trimethoprim
-
 Wuchereria bancrofti
1.5.1.3 vitexin
-
 Brugia malayi
1.5.1.3 vitexin
-
 Wuchereria bancrofti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0037
-
 7,8-dihydrofolate pH 6, 25°C Wuchereria bancrofti
1.5.1.3 0.0147
-
 7,8-dihydrofolate pH 6, 25°C Brugia malayi

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Brugia malayi
-
-
-
1.5.1.3 Wuchereria bancrofti J9F199 sequence lacks a crucial, conserved 13 amino acid loop
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 recombinant protein, using Ni-NTA resin and imidazole concentration in the wash buffer is changed from 25 mM to 100 mM Brugia malayi
1.5.1.3 recombinant protein, using Ni-NTA resin and imidazole concentration in the wash buffer is changed from 25 mM to 100 mM Wuchereria bancrofti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Brugia malayi 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Wuchereria bancrofti 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 WUBG_00817
-
 Wuchereria bancrofti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 2.2
-
 7,8-dihydrofolate pH 6, 25°C Brugia malayi
1.5.1.3 7.4
-
 7,8-dihydrofolate pH 6, 25°C Wuchereria bancrofti

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.0000005
-
 methotrexate pH 6, 25°C Brugia malayi
1.5.1.3 0.0000007
-
 methotrexate pH 6, 25°C Wuchereria bancrofti
1.5.1.3 0.0000017
-
 aminopterin pH 6, 25°C Brugia malayi
1.5.1.3 0.0000021
-
 aminopterin pH 6, 25°C Wuchereria bancrofti
1.5.1.3 0.0016
-
 raltitrexed pH 6, 25°C Brugia malayi
1.5.1.3 0.002
-
 raltitrexed pH 6, 25°C Wuchereria bancrofti
1.5.1.3 0.0036
-
 pyrimethamine pH 6, 25°C Brugia malayi
1.5.1.3 0.00598
-
 trimethoprim pH 6, 25°C Wuchereria bancrofti
1.5.1.3 0.015
-
 trimethoprim pH 6, 25°C Brugia malayi
1.5.1.3 0.015
-
 pyrimethamine pH 6, 25°C Wuchereria bancrofti

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.3 additional information
-
 value above 1 mM, pH 6, 25°C Brugia malayi (-)-epicatechin gallate
1.5.1.3 additional information
-
 value above 1 mM, pH 6, 25°C Wuchereria bancrofti (-)-epicatechin gallate
1.5.1.3 additional information
-
 value above 2.5 mM, pH 6, 25°C Brugia malayi (-)-epicatechin
1.5.1.3 additional information
-
 value above 2.5 mM, pH 6, 25°C Wuchereria bancrofti (-)-epicatechin
1.5.1.3 0.0000022
-
 pH 6, 25°C Brugia malayi methotrexate
1.5.1.3 0.0000075
-
 pH 6, 25°C Brugia malayi aminopterin
1.5.1.3 0.000014
-
 pH 6, 25°C Wuchereria bancrofti aminopterin
1.5.1.3 0.000018
-
 pH 6, 25°C Wuchereria bancrofti methotrexate
1.5.1.3 0.0073
-
 pH 6, 25°C Brugia malayi raltitrexed
1.5.1.3 0.016
-
 pH 6, 25°C Brugia malayi pyrimethamine
1.5.1.3 0.018
-
 pH 6, 25°C Wuchereria bancrofti raltitrexed
1.5.1.3 0.065
-
 pH 6, 25°C Brugia malayi trimethoprim
1.5.1.3 0.083
-
 pH 6, 25°C Wuchereria bancrofti trimethoprim
1.5.1.3 0.24
-
 pH 6, 25°C Brugia malayi vitexin
1.5.1.3 0.24
-
 pH 6, 25°C Wuchereria bancrofti vitexin
1.5.1.3 0.454
-
 pH 6, 25°C Wuchereria bancrofti pyrimethamine