Literature summary extracted from
Wever, R.; Krenn, B.; Renirie, R.
Marine vanadium-dependent haloperoxidases, their isolation, characterization, and application (2018), Methods Enzymol., 605, 141-201 .
Application
EC Number |
Application |
Comment |
Organism |
---|
1.11.1.B6 |
analysis |
for iodoperoxidase activity measurement, the triiodide assay should only be used for qualitative applications. A quantitative spectrophotometric assay is based on the iodination of thymol blue but can only be used in the pH range 7-8 |
More |
1.11.1.B6 |
analysis |
to detect activity on polyacrylamide gels or in cross sections of algal part, o-dianisidine may be used. The probes are first incubated in a buffered solution containing 100 mM KBr or 100 mM KI, and 10 mM o-dianisidine, and the staining is started by addition of 2 mM H2O2. The formed HOBr or HOI will oxidize the organic dye resulting in polymerization and formation of a brown precipitate |
More |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.11.1.B6 |
More |
- |
vanadium-dependent haloperoxidases in seaweeds, cyanobacteria, fungi, and phytoplankton |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.11.1.B6 |
purification of enzyme from seaweeds, overview |
More |