Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guan, Z.B.; Luo, Q.; Wang, H.R.; Chen, Y.; Liao, X.R.
    Bacterial laccases promising biological green tools for industrial applications (2018), Cell. Mol. Life Sci., 75, 3569-3592 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection treating waste water containing synthetic dyes Bacillus subtilis
1.10.3.2 additional information pulp biobleaching Bacillus tequilensis
1.10.3.2 paper production biobleaching of eucalyptus kraft pulps results in a significant decrease in the kappa number and a significant increase in the brightness of the pulps Streptomyces cyaneus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression in Escherichia coli Bacillus subtilis
1.10.3.2 expression in Escherichia coli Bacillus pumilus
1.10.3.2 expression in Escherichia coli Bacillus vallismortis
1.10.3.2 expression in Escherichia coli Streptomyces coelicolor
1.10.3.2 expression in Escherichia coli Streptomyces cyaneus
1.10.3.2 expression in Escherichia coli or in Pichia pastoris Bacillus licheniformis

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.2 D500G in Pichia pastoris 9.3folds higher expression than wild-type enzyme Bacillus licheniformis
1.10.3.2 D501G better stability and catalytic efficiency than wild-type enzyme Bacillus amyloliquefaciens
1.10.3.2 E106F mutation promotes both enzymatic activity and thermostability Shigella dysenteriae
1.10.3.2 K316N 11.4-fold higher expression level. High dimerization of phenolic and decolorization of industrial dyes Bacillus licheniformis
1.10.3.2 Y108A Tyr108 does form an integral part of the active site and affects enzyme kinetics Streptomyces coelicolor
1.10.3.2 Y108F Tyr108 does form an integral part of the active site and affects enzyme kinetics Streptomyces coelicolor
1.10.3.2 Y229A over 10fold increase in activity Streptomyces coelicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
 Bacillus tequilensis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper multicopper enzyme Bacillus subtilis
1.10.3.2 copper multicopper enzyme Lysinibacillus sphaericus
1.10.3.2 copper multicopper enzyme Bacillus cereus
1.10.3.2 copper multicopper enzyme Bacillus amyloliquefaciens
1.10.3.2 copper multicopper enzyme Bacillus pumilus
1.10.3.2 copper multicopper enzyme Streptomyces griseus
1.10.3.2 copper multicopper enzyme Streptomyces bikiniensis
1.10.3.2 copper multicopper enzyme Kitasatospora aureofaciens
1.10.3.2 copper multicopper enzyme Streptomyces lividans
1.10.3.2 copper multicopper enzyme Shigella dysenteriae
1.10.3.2 copper multicopper enzyme Halalkalibacterium halodurans
1.10.3.2 copper multicopper enzyme Alkalihalobacillus clausii
1.10.3.2 copper multicopper enzyme Bacillus safensis
1.10.3.2 copper multicopper enzyme Streptomyces violaceusniger
1.10.3.2 copper multicopper enzyme Bacillus tequilensis
1.10.3.2 copper multicopper enzyme Bacillus altitudinis
1.10.3.2 copper multicopper enzyme Bacillus licheniformis
1.10.3.2 copper multicopper enzyme Bacillus vallismortis
1.10.3.2 copper multicopper enzyme Streptomyces coelicolor
1.10.3.2 copper multicopper enzyme Streptomyces cyaneus
1.10.3.2 copper multicopper enzyme Streptomyces lavendulae
1.10.3.2 copper multicopper enzyme Streptomyces ipomoeae
1.10.3.2 copper multicopper enzyme Streptomyces viridosporus
1.10.3.2 copper multicopper enzyme Streptomyces sviceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 58500
-
-
 Bacillus subtilis
1.10.3.2 65000
-
-
 Bacillus pumilus
1.10.3.2 69500
-
-
 Streptomyces cyaneus
1.10.3.2 70000
-
-
 Bacillus vallismortis

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Alkalihalobacillus clausii
-
-
-
1.10.3.2 Bacillus altitudinis
-
-
-
1.10.3.2 Bacillus amyloliquefaciens
-
-
-
1.10.3.2 Bacillus cereus
-
-
-
1.10.3.2 Bacillus licheniformis A0A6B9ET10
-
-
1.10.3.2 Bacillus pumilus
-
-
-
1.10.3.2 Bacillus pumilus I3RYX9
-
-
1.10.3.2 Bacillus pumilus MK001 I3RYX9
-
-
1.10.3.2 Bacillus pumilus W3
-
-
-
1.10.3.2 Bacillus safensis
-
-
-
1.10.3.2 Bacillus subtilis
-
-
-
1.10.3.2 Bacillus subtilis C6KEH7
-
-
1.10.3.2 Bacillus subtilis P07788
-
-
1.10.3.2 Bacillus subtilis 168 P07788
-
-
1.10.3.2 Bacillus subtilis cjp3
-
-
-
1.10.3.2 Bacillus subtilis WD23 C6KEH7
-
-
1.10.3.2 Bacillus tequilensis
-
-
-
1.10.3.2 Bacillus tequilensis SN4
-
-
-
1.10.3.2 Bacillus vallismortis S5MW00
-
-
1.10.3.2 Bacillus vallismortis fmb-103 S5MW00
-
-
1.10.3.2 Halalkalibacterium halodurans
-
-
-
1.10.3.2 Kitasatospora aureofaciens
-
-
-
1.10.3.2 Lysinibacillus sphaericus
-
-
-
1.10.3.2 Shigella dysenteriae
-
-
-
1.10.3.2 Streptomyces bikiniensis
-
-
-
1.10.3.2 Streptomyces coelicolor Q9XAL8
-
-
1.10.3.2 Streptomyces coelicolor ATCC BAA-471 Q9XAL8
-
-
1.10.3.2 Streptomyces cyaneus F6L7B5
-
-
1.10.3.2 Streptomyces cyaneus CECT 3335 F6L7B5
-
-
1.10.3.2 Streptomyces griseus
-
-
-
1.10.3.2 Streptomyces ipomoeae A3QVH2
-
-
1.10.3.2 Streptomyces ipomoeae CECT 3341 A3QVH2
-
-
1.10.3.2 Streptomyces lavendulae Q8GB87
-
-
1.10.3.2 Streptomyces lividans
-
-
-
1.10.3.2 Streptomyces sviceus
-
-
-
1.10.3.2 Streptomyces violaceusniger
-
-
-
1.10.3.2 Streptomyces viridosporus J9PBR2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 endospore
-
 Bacillus licheniformis
-
1.10.3.2 endospore spore-bound Bacillus subtilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus subtilis ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus vallismortis ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Streptomyces cyaneus ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus W3 ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus subtilis cjp3 ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus vallismortis fmb-103 ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Streptomyces cyaneus CECT 3335 ?
-
 ?
1.10.3.2 2,6-dimethylphthalate + O2
-
 Streptomyces cyaneus ?
-
 ?
1.10.3.2 2,6-dimethylphthalate + O2
-
 Streptomyces cyaneus CECT 3335 ?
-
 ?
1.10.3.2 acetosyringone + O2
-
 Bacillus vallismortis ?
-
 ?
1.10.3.2 acetosyringone + O2
-
 Bacillus vallismortis fmb-103 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Streptomyces cyaneus 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
1.10.3.2 guaiacol + O2
-
 Streptomyces cyaneus CECT 3335 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
1.10.3.2 Malachite Green + O2
-
 Bacillus vallismortis ?
-
 ?
1.10.3.2 Malachite Green + O2
-
 Bacillus vallismortis fmb-103 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus subtilis ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus vallismortis ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus subtilis cjp3 ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus vallismortis fmb-103 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus subtilis
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Lysinibacillus sphaericus
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus cereus
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus amyloliquefaciens
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus pumilus
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces griseus
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces bikiniensis
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Kitasatospora aureofaciens
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces lividans
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Shigella dysenteriae
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Halalkalibacterium halodurans
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Alkalihalobacillus clausii
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus safensis
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces violaceusniger
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus tequilensis
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus altitudinis
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus licheniformis
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Bacillus vallismortis
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces coelicolor
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces cyaneus
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces lavendulae
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces ipomoeae
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces viridosporus
1.10.3.2 Benzenediol:oxygen oxidoreductase
-
 Streptomyces sviceus
1.10.3.2 CotA-laccase
-
 Bacillus subtilis
1.10.3.2 CotA-laccase
-
 Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 80
-
-
 Bacillus subtilis
1.10.3.2 80
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus pumilus
1.10.3.2 80
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Streptomyces cyaneus
1.10.3.2 85
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus vallismortis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 20 80 stable from 20°C-80°C Bacillus subtilis
1.10.3.2 50
-
 6 h, 50% loss of activity Bacillus pumilus
1.10.3.2 60
-
 1 h, 50% loss of activity Streptomyces cyaneus
1.10.3.2 70
-
 half-life: 100 min Streptomyces lavendulae
1.10.3.2 70
-
 10 h, more than 50% of activity Bacillus vallismortis
1.10.3.2 80
-
 half-life: 1 h Bacillus pumilus
1.10.3.2 80
-
 half-life: 2.5 h Bacillus subtilis
1.10.3.2 80
-
 half-life: 1.14 h Bacillus pumilus
1.10.3.2 80
-
 half-life: 112 min Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3.6
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus pumilus
1.10.3.2 4.8
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus vallismortis
1.10.3.2 5
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus subtilis
1.10.3.2 5.5
-
 substrate: 2,6-dimethylphthalate Streptomyces cyaneus
1.10.3.2 7.4
-
 substrate: syringaldazine Bacillus vallismortis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 additional information
-
 stable at alkaline pH Bacillus pumilus
1.10.3.2 5.5
-
 24 h, 40% of activity Streptomyces cyaneus
1.10.3.2 6.8
-
 half-life: 6 months Bacillus subtilis
1.10.3.2 9
-
 half-life: 15 days Bacillus subtilis
1.10.3.2 9
-
 stable for 10 h Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 1.72
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH and temperature not specified in the publication Streptomyces cyaneus
1.10.3.2 4.737
-
 2,6-dimethylphthalate pH and temperature not specified in the publication Streptomyces cyaneus
1.10.3.2 5.17
-
 guaiacol pH and temperature not specified in the publication Streptomyces cyaneus
1.10.3.2 157.86
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH and temperature not specified in the publication Bacillus pumilus