Literature summary extracted from

Liu, J.X.; Feng, K.; Duan, A.Q.; Li, H.; Yang, Q.Q.; Xu, Z.S.; Xiong, A.S.
Isolation, purification and characterization of an ascorbate peroxidase from celery and overexpression of the AgAPX1 gene enhanced ascorbate content and drought tolerance in Arabidopsis (2019), BMC Plant Biol., 19, 488 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.11.1.11	gene AgAPX1, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), recombinant GFP-tagged enzyme expression in onion epidermal cells for analysis of subcellular localization of AgAPX1. Overexpression of AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture. Net photosynthetic rate considerably decreases in the wild-type compared to transgenic lines under drought stress	Apium graveolens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.11.1.11	cytosol	-	Apium graveolens	5829	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.11.1.11	Fe2+	in the heme group	Apium graveolens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.11.1.11	2 L-ascorbate + H2O2 + 2 H+	Apium graveolens	-	L-ascorbate + L-dehydroascorbate + 2 H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.11.1.11	Apium graveolens	-	cv. Jinnanshiqin	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.11.1.11	recombinant enzyme from Escherichia coli strain BL21(DE3)	Apium graveolens

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.11.1.11	leaf	-	Apium graveolens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.11.1.11	2 L-ascorbate + H2O2 + 2 H+	-	Apium graveolens	L-ascorbate + L-dehydroascorbate + 2 H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.11.1.11	?	x * 33160, recombinant enzyme, sequence calculation, x * 34000, recombinant enzyme, SDS-PAGE	Apium graveolens

Synonyms

EC Number	Synonyms	Comment	Organism
1.11.1.11	ascorbate peroxidase	-	Apium graveolens

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.11.1.11	55	-	recombinant enzyme	Apium graveolens

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
1.11.1.11	20	85	activity range, profile overview. Inactivation at 90°C, maximum activity at 55°C	Apium graveolens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.11.1.11	7	-	recombinant enzyme	Apium graveolens

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.11.1.11	4.5	9.5	activity range, profile overview	Apium graveolens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.11.1.11	heme	-	Apium graveolens

General Information

EC Number	General Information	Comment	Organism
1.11.1.11	evolution	sequence comparison, phylogenetic analysis and tree	Apium graveolens
1.11.1.11	physiological function	recombinant overexpression of celery enzyme AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture, physiological changes in Arabidopsis leaves exposed to drought stress, phenotype, overview. The AgAPX1 gene seems to be involved in the response of celery to drought stress. The response of the AgAPX1 gene to adversity may be attributed to the conservation of APX sequences among different species	Apium graveolens

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Release 2023.1 (January 2023)