Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reddy, V.A.; Li, C.; Nadimuthu, K.; Tjhang, J.G.; Jang, I.C.; Rajani, S.
    Sweet basil has distinct synthases for eugenol biosynthesis in glandular trichomes and roots with different regulatory mechanisms (2021), Int. J. Mol. Sci., 22, 681 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.318 gene EGS1, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4 Ocimum basilicum
1.1.1.318 gene EGS2, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter Ocimum basilicum
1.1.1.318 gene EGS3, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter Ocimum basilicum
1.1.1.318 gene EGS4, genotyping and quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter. Analysis of ObEGS1 and ObEGS4 endogenous promoters shows the presence of different cis regulatory elements, expression pattern. An AC-II element is found in ObEGS1 promoter and a MYB biding site (MBS) is found in ObEGS4 Ocimum basilicum
1.1.1.318 gene EGS5, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter Ocimum basilicum
1.1.1.318 gene EGS6, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter Ocimum basilicum
1.1.1.318 gene EGS7, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter Ocimum basilicum
1.1.1.318 gene EGS8, genotyping and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant Agrobacterium-mediated expression of YFP-tagged isozyme in Nicotiana benthamiana leaf cell cytosol under control of the CaMV35S promoter Ocimum basilicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.318 cytosol
-
 Ocimum basilicum 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.318 coniferyl acetate + NADPH + H+ Ocimum basilicum
-
 eugenol + acetate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.318 Ocimum basilicum
-
-
-
1.1.1.318 Ocimum basilicum A0A1B2U6R8
-
-
1.1.1.318 Ocimum basilicum A0A1B2U6S4
-
-
1.1.1.318 Ocimum basilicum A0A1B2U6T3
-
-
1.1.1.318 Ocimum basilicum A0A7S9C1H0
-
-
1.1.1.318 Ocimum basilicum A0A7S9C1M1
-
-
1.1.1.318 Ocimum basilicum A0A7U3PJH6
-
-
1.1.1.318 Ocimum basilicum Q15GI4
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.318 additional information posttranscriptional modifications can regulate eugenol synthase in the roots of sweet basil Ocimum basilicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.318 leaf
-
 Ocimum basilicum
-
1.1.1.318 leaf leaves and leaves stripped of PGTs Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS1 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves, not in roots and leaves stripped of PGTs Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS2 is mainly expressed in peltate glandular trichomes (PGTs) and only very weakly in leaves and roots, but not in leaves stripped of PGTs Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS3 is expressed in peltate glandular trichomes (PGTs) and roots, but not in leaves and leaves stripped of PGTs Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS4 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS5 is expressed in all tissues analyzed with highest expression levels in peltate glandular trichomes, followed by roots, and very low expression levels in all leaves Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS6 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs) and leaves, but no expression in leaves stripped of PGTs Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS7 is expressed in all tissues analyzed with highest expression levels in leaves and leaves stripped of peltate glandular trichomes Ocimum basilicum
-
1.1.1.318 additional information isozyme expression analysis in different tissues, transcriptome analysis, overview. Isozyme EGS8 is mainly expressed in roots, it also shows weak expression in peltate glandular trichomes (PGTs), but no expression in leaves and leaves stripped of PGTs Ocimum basilicum
-
1.1.1.318 peltate glandular trichome PGT Ocimum basilicum
-
1.1.1.318 root the amount of eugenol produced in roots of aseptically-grown plants under elicitation is much lower than the amount produced in roots of soil-grown plants. This might be because of the different external factors and multiple stress conditions experienced in soil as compared to aseptic conditions Ocimum basilicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.318 4-coumaryl acetate + NADPH + H+
-
 Ocimum basilicum chavicol + acetate + NADP+
-
 r
1.1.1.318 coniferyl acetate + NADPH + H+
-
 Ocimum basilicum eugenol + acetate + NADP+
-
 r
1.1.1.318 additional information GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes Ocimum basilicum ?
-
-
1.1.1.318 additional information no activity with 4-coumaryl acetate. GC-MS analysis of products formed in Escherichia coli recombinantly expressing ObEGS isozymes Ocimum basilicum ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.318 eugenol synthase 1
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 2
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 3
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 4
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 5
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 6
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 7
-
 Ocimum basilicum
1.1.1.318 eugenol synthase 8
-
 Ocimum basilicum
1.1.1.318 ObEGS1
-
 Ocimum basilicum
1.1.1.318 ObEGS2
-
 Ocimum basilicum
1.1.1.318 ObEGS3
-
 Ocimum basilicum
1.1.1.318 ObEGS4
-
 Ocimum basilicum
1.1.1.318 ObEGS5
-
 Ocimum basilicum
1.1.1.318 ObEGS6
-
 Ocimum basilicum
1.1.1.318 ObEGS7
-
 Ocimum basilicum
1.1.1.318 ObEGS8
-
 Ocimum basilicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.318 NADP+
-
 Ocimum basilicum
1.1.1.318 NADPH
-
 Ocimum basilicum

Expression

EC Number Organism Comment Expression
1.1.1.318 Ocimum basilicum methyl jasmonate, pectinase, and infection by a fungus can induce eugenol production in aseptically grown roots up
1.1.1.318 Ocimum basilicum methyl jasmonate, pectinase, and infection by fungus can induce eugenol production in aseptically grown roots up

General Information

EC Number General Information Comment Organism
1.1.1.318 evolution Ocimum basilicum contains 8 isozymes of eugenol synthase, EGS. The enzymes belong to the PIP family, named after the first three identified members, pinoresinollariciresinol reductase (PLR), isoflavone reductase (IFR), and phenylcoumaran benzylic ether reductase (PCBER) Ocimum basilicum