Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Im, D.; Matsui, D.; Arakawa, T.; Isobe, K.; Asano, Y.; Fushinobu, S.
    Ligand complex structures of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 and its conformational change (2018), FEBS open bio, 8, 314-324 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.2 structures of L-AAO/MOG complexed with L-Lys, L-ornithine, and L-Arg. Residue Asp238 is located at the ceiling of a long hydrophobic pocket and forms a strong interaction with the terminal, positively charged group of the substrates. In the ligand-free structure, there are two channels connecting the active site and solvent, and a short region located at the dimer interface is disordered. In the L-Lys complex structure, a loop region is displaced to plug the channels Pseudomonas sp.
1.13.12.2 structures of L-AAO/MOG complexed with L-Lys, L-ornithine, and L-Arg. Residue Asp238 is located at the ceiling of a long hydrophobic pocket and forms a strong interaction with the terminal, positively charged group of the substrates. In the ligand-free structure, there are two channels connecting the active site and solvent, and a short region located at the dimer interface is disordered. In the L-Lys complex structure, a loop region is displaced to plug the channels Pseudomonas sp. AIU 813

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.2 D238A the interaction of Asp238 with the terminal, positively charged group of the substratesis critical for substrate binding but not for catalytic control between the oxidase/monooxygenase activities Pseudomonas sp.
1.4.3.2 D238E mutant displays increased catalytic activities Pseudomonas sp.
1.4.3.2 D238F mutant exhibits altered substrate specificity to long hydrophobic substrates Pseudomonas sp.
1.13.12.2 D238A the interaction of Asp238 with the terminal, positively charged group of the substrates is critical for substrate binding but not for catalytic control between the oxidase/monooxygenase activities Pseudomonas sp. AIU 813
1.13.12.2 D238E mutant displays increased catalytic activities Pseudomonas sp. AIU 813
1.13.12.2 D238F mutant exhibits altered substrate specificity to long hydrophobic substrates Pseudomonas sp. AIU 813

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.018
-
 L-lysine mutant D238E, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.022
-
 L-ornithine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.027
-
 L-lysine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.041
-
 L-ornithine mutant D238E, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.048
-
 L-arginine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.059
-
 L-lysine mutant D238F, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.08
-
 L-arginine mutant D238F, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.41
-
 L-arginine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.45
-
 L-ornithine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.61
-
 L-lysine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.13.12.2 0.033
-
 L-ornithine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.043
-
 L-lysine mutant D238E, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.061
-
 L-lysine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.061
-
 L-ornithine mutant D238E, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.067
-
 L-arginine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.072
-
 L-lysine mutant D238F, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.09
-
 L-arginine mutant D238F, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.26
-
 L-lysine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.31
-
 L-ornithine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.42
-
 L-arginine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Pseudomonas sp. W6JQJ6 bifunctional L-amino acid oxidase/monooxygenase, cf. EC. 1.13.12.2
-
1.13.12.2 Pseudomonas sp. AIU 813 W6JQJ6 bifunctional L-amino acid oxidase/monooxygenase, cf. EC. 1.4.3.2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-arginine + H2O + O2
-
 Pseudomonas sp. 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-lysine + H2O + O2
-
 Pseudomonas sp. 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-ornithine + H2O + O2
-
 Pseudomonas sp. 5-amino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.13.12.2 L-arginine + O2
-
 Pseudomonas sp. AIU 813 4-guanidinobutanamide + CO2 + H2O
-
 ?
1.13.12.2 L-lysine + O2
-
 Pseudomonas sp. AIU 813 5-aminopentanamide + CO2 + H2O
-
 ?
1.13.12.2 L-ornithine + O2
-
 Pseudomonas sp. AIU 813 4-aminobutanamide + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-AAO/MOG
-
 Pseudomonas sp.
1.13.12.2 L-AAO/MOG
-
 Pseudomonas sp. AIU 813

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 0.1
-
 L-arginine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.1
-
 L-arginine mutant D238F, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.17
-
 L-lysine mutant D238F, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.37
-
 L-ornithine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.41
-
 L-arginine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.5
-
 L-lysine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.58
-
 L-ornithine mutant D238E, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.8
-
 L-lysine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 1.5
-
 L-ornithine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 2.3
-
 L-lysine mutant D238E, pH 7, 30°C Pseudomonas sp.
1.13.12.2 0.29
-
 L-ornithine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 0.8
-
 L-lysine mutant D238F, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 1.2
-
 L-arginine mutant D238F, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 1.6
-
 L-arginine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 2.3
-
 L-ornithine mutant D238E, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 2.7
-
 L-lysine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 2.8
-
 L-arginine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 4.5
-
 L-lysine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 6.7
-
 L-ornithine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 8.1
-
 L-lysine mutant D238E, pH 7, 30°C Pseudomonas sp. AIU 813

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.2 0.24
-
 L-arginine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.82
-
 L-lysine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 0.82
-
 L-ornithine mutant D238A, pH 7, 30°C Pseudomonas sp.
1.4.3.2 1.3
-
 L-arginine mutant D238F, pH 7, 30°C Pseudomonas sp.
1.4.3.2 3.5
-
 L-lysine mutant D238F, pH 7, 30°C Pseudomonas sp.
1.4.3.2 8.5
-
 L-arginine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 14
-
 L-ornithine mutant D238E, pH 7, 30°C Pseudomonas sp.
1.4.3.2 30
-
 L-lysine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 68
-
 L-ornithine wild-type, pH 7, 30°C Pseudomonas sp.
1.4.3.2 130
-
 L-lysine mutant D238E, pH 7, 30°C Pseudomonas sp.
1.13.12.2 0.94
-
 L-ornithine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 6.7
-
 L-arginine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 10
-
 L-lysine mutant D238A, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 11
-
 L-lysine mutant D238F, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 13
-
 L-arginine mutant D238F, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 24
-
 L-arginine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 32
-
 L-ornithine mutant D238E, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 74
-
 L-lysine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 190
-
 L-lysine mutant D238E, pH 7, 30°C Pseudomonas sp. AIU 813
1.13.12.2 200
-
 L-ornithine wild-type, pH 7, 30°C Pseudomonas sp. AIU 813