Literature summary extracted from

Zhan, T.; Chen, Q.; Zhang, C.; Bi, C.; Zhang, X.
Constructing a novel biosynthetic pathway for the production of glycolate from glycerol in Escherichia coli (2020), ACS Synth. Biol., 9, 2600-2609 .

Application

EC Number	Application	Comment	Organism
1.1.3.41	synthesis	biosynthetic production of glycolate from glycerol using a variant of alditol oxidase, 2-hydroxyglutarate-pyruvate transhydrogenase from Saccharomyces cerevisiae, alpha-ketoisovalerate decarboxylase from Lactococcus lactis, and aldehyde dehydrogenase from Escherichia coli in an artificial operon expressed in Escherichia coli. To redirect glycerol flux toward glycolate synthesis, key genes of the native glycerol assimilation pathways are deleted and a second plasmid expressing Dld3 to reduce the accumulation of the intermediate D-glycerate is introduced. The final engineered strain produces 0.64 g/l glycolate in shake flasks, which is increased to 4.74 g/l in fed-batch fermentation	Streptomyces coelicolor

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.3.41	Streptomyces coelicolor	-	-	-
1.1.3.41	Streptomyces coelicolor A3(2)	-	-	-

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Release 2023.1 (January 2023)