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Literature summary extracted from

  • Sharma, R.; Devi, K.; Modi, M.K.; Sen, P.
    In silico characterization and differential expression analysis of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) of Centella asiatica (2021), 3 Biotech, 11, 184 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 gene DXR, sequence comparisons and phylogenetic analysis and tree, semi-quantitative RT-PCR and quantitative real-time PCR expression analysis, recombinant expression Centella asiatica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.267 chloroplast the enzyme sequence contains a conserved transit peptide for plastid motif Cys-Ser-(Ala/Met/Val/Thr) Centella asiatica 9507
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1.1.1.267 additional information Centella DXR transcript is localized in chloroplasts and other plastids Centella asiatica
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Centella asiatica
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 2-C-methyl-D-erythritol 4-phosphate + NADP+
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Centella asiatica A0A0A6Z621
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-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.267 leaf
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 Centella asiatica
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1.1.1.267 additional information CaDXR is differentially expressed in different tissues with maximal expression in the node and lowest in the roots, Centella DXR transcript is expressed in vegetative tissues Centella asiatica
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1.1.1.267 node high expression level Centella asiatica
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1.1.1.267 root low expression level Centella asiatica
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1.1.1.267 stem
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 Centella asiatica
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
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 Centella asiatica 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.267 ? x * 51500, SDS-PAGE Centella asiatica
1.1.1.267 More enzyme CaDXR contains three conserved domain, namely NADPH (GSTGSIGT and LAAGSNV), substrate binding (LPADSEHSAI and NKGLEVIEAHY) and Cys-Ser-(Ala/Met/Val/Thr) cleavage-site domains. CaDXR is a basically alpha-helical protein which contains 21 alpha-helixes, 11 beta-sheets, and one hairpin structure Centella asiatica

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 CaDXR
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 Centella asiatica
1.1.1.267 DXR
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 Centella asiatica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 additional information cofactor docking study with DXR, presence of conserved NADPH-binding motif (GSTGSIGT and LAAGSNV) in N-terminal end of the CaDXR protein sequence Centella asiatica
1.1.1.267 NADP+
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 Centella asiatica
1.1.1.267 NADPH
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 Centella asiatica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.267 Centella asiatica isoelectric focusing
-
 6.33

General Information

EC Number General Information Comment Organism
1.1.1.267 metabolism the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) is the rate-limiting enzyme in the methylerythritol 4-phosphate pathway (MEP) via conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) into MEP Centella asiatica
1.1.1.267 additional information enzyme CaDXR contains three conserved domain, namely NADPH (GSTGSIGT and LAAGSNV), substrate binding (LPADSEHSAI and NKGLEVIEAHY) and Cys-Ser-(Ala/Met/Val/Thr) cleavage-site domains. Three-dimensional molecular modeling and model validation, and molecular dynamics simulation of modeled DXR, overview Centella asiatica
1.1.1.267 physiological function the 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), an NADPH-dependent reductase, plays a pivotal role in the methylerythritol 4-phosphate pathway (MEP), in the conversion of 1-deoxy-D-xylulose-5-phosphate (DXP) into MEP. Structure-function analysis of DXR and regulatory mechanism, molecular dynamics simulations, overview Centella asiatica