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Literature summary extracted from

  • Jiang, L.; Chen, Y.; Zheng, J.; Chen, Z.; Liu, Y.; Tao, Y.; Wu, W.; Chen, Z.; Wang, B.
    Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein (2016), Plant Physiol., 170, 732-741 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.32 expressed in Escherichia coli BL21(DE3) RIL cells Zea mays

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.11.32 using 1.0 M K/Na tartrate 0.1 M MOPS, pH 8.0, 1% (v/v) dioxane, 1 mM AMP, and 2 mM Mg2 Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.32 C128S the activity if the mutant is comparable to that of the wild type enzyme Zea mays
2.7.11.32 C165S the activity if the mutant is comparable to that of the wild type enzyme Zea mays
2.7.11.32 D276A/D277A the mutant exhibits a marked decrease in phosphorylation and dephosphorylation activities as compared to the wild type enzyme Zea mays
2.7.11.32 K274A the mutation exhibits little effect on phosphorylation and dephosphorylation activities as compared to the wild type enzyme Zea mays
2.7.11.32 P317F the mutant exhibits a marked decrease in phosphorylation and dephosphorylation activities as compared to the wild type enzyme Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.32 Mg2+ 10 mM Mg2+ use din assay conditions Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.32 ADP + [pyruvate phosphate dikinase] Zea mays
-
 AMP + [pyruvate phosphate dikinase] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.32 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.32 Imac resin column chromatography Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.32 ADP + [pyruvate phosphate dikinase]
-
 Zea mays AMP + [pyruvate phosphate dikinase] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.32 homodimer at pH 7.5. The enzyme mainly exists as a dimer Zea mays
2.7.11.32 homotetramer at pH 8.3 Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.11.32 PDRP
-
 Zea mays
2.7.11.32 PPDK regulatory protein
-
 Zea mays
2.7.11.32 pyruvate orthophosphate dikinase regulatory protein
-
 Zea mays

General Information

EC Number General Information Comment Organism
2.7.11.32 metabolism the enzyme regulates the inorganic phosphate-dependent activation and ADP-dependent inactivation of pyruvate phosphate dikinase by reversible phosphorylation Zea mays