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Literature summary extracted from

  • Alvarez, C.E.; Bovdilova, A.; Hoeppner, A.; Wolff, C.C.; Saigo, M.; Trajtenberg, F.; Zhang, T.; Buschiazzo, A.; Nagel-Steger, L.; Drincovich, M.F.; Lercher, M.J.; Maurino, V.G.
    Molecular adaptations of NADP-malic enzyme for its function in C4 photosynthesis in grasses (2019), Nat. Plants, 5, 755-765 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.40 hanging drop vapor diffusion method, using 0.2 M potassium fluoride and 30% (w/v) PEG 3350 Sorghum bicolor
1.1.1.40 sitting drop vapor diffusion method, using 0.1 M potassium chloride, 0.1 M sodium citrate (pH 5.5) and 34% (w/v) pentaerythritol propoxylate Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.40 A339E the mutant of isoform nonC4-NADP-ME shows increased catalytic efficiency compared to the wild type enzyme Zea mays
1.1.1.40 DelN the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
1.1.1.40 DelN/A339E the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays
1.1.1.40 DelN/I140F/A339E the mutant of isoform nonC4-NADP-ME shows increased catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
1.1.1.40 E339A the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
1.1.1.40 F140I the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays
1.1.1.40 I140F the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
1.1.1.40 I140F/A339E the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
1.1.1.40 L544F the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays
1.1.1.40 Q503E the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.40 (S)-malate
-
 Sorghum bicolor
1.1.1.40 (S)-malate
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.0082
-
 NADP+ mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.0128
-
 NADP+ mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.0131
-
 NADP+ mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.0149
-
 NADP+ wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.015
-
 NADP+ mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.0159
-
 NADP+ mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.0159
-
 NADP+ mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.023
-
 NADP+ mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.023
-
 NADP+ wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.038
-
 NADP+ mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.0412
-
 NADP+ mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.0503
-
 NADP+ mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.23
-
 (S)-malate wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.27
-
 (S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.4
-
 (S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.43
-
 (S)-malate wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.46
-
 (S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.47
-
 (S)-malate mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.47
-
 (S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.58
-
 (S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.85
-
 (S)-malate mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 0.95
-
 (S)-malate mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 1.05
-
 (S)-malate mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 1.12
-
 (S)-malate mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 chloroplast
-
 Zea mays 9507
-
1.1.1.40 chloroplast
-
 Sorghum bicolor 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Zea mays
-
 pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 (S)-malate + NADP+ Sorghum bicolor
-
 pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+ Zea mays
-
 (S)-malate + NADP+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+ Sorghum bicolor
-
 (S)-malate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Sorghum bicolor
-
 cultivar v.3.1.1
-
1.1.1.40 Zea mays
-
 cultivar Ensembl-18
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 Ni-NTA agarose column chromatography Zea mays
1.1.1.40 Ni-NTA agarose column chromatography Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Zea mays pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 (S)-malate + NADP+
-
 Sorghum bicolor pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+
-
 Zea mays (S)-malate + NADP+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+
-
 Sorghum bicolor (S)-malate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.40 homotetramer 4 * 66000, SDS-PAGE Zea mays
1.1.1.40 homotetramer 4 * 66000, SDS-PAGE Sorghum bicolor

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 C4-NADP-ME isoform Zea mays
1.1.1.40 C4-NADP-ME isoform Sorghum bicolor
1.1.1.40 NADP-malic enzyme
-
 Zea mays
1.1.1.40 NADP-malic enzyme
-
 Sorghum bicolor
1.1.1.40 nicotinamide adenine dinucleotide phosphate-dependent malic enzyme
-
 Zea mays
1.1.1.40 nicotinamide adenine dinucleotide phosphate-dependent malic enzyme
-
 Sorghum bicolor
1.1.1.40 nonC4-NADP-ME isoform Zea mays
1.1.1.40 nonC4-NADP-ME isoform Sorghum bicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 3.6
-
 (S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 4
-
 (S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 22
-
 (S)-malate mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 26.3
-
 (S)-malate wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 27.4
-
 (S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 28.1
-
 (S)-malate wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 28.7
-
 (S)-malate mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 29.8
-
 (S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 32.1
-
 (S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 36.7
-
 (S)-malate mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 39.6
-
 (S)-malate mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 85.8
-
 (S)-malate mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 7.5 8
-
 Zea mays
1.1.1.40 7.5 8
-
 Sorghum bicolor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.40 9.1
-
 (S)-malate wild type isoform C4-NADP-ME, at pH 8.0 and 25°C Zea mays
1.1.1.40 13.3
-
 (S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 13.4
-
 (S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 15.8
-
 (S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 16.7
-
 (S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 19.4
-
 (S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.40 7.7
-
 (S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 10
-
 (S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 23.2
-
 (S)-malate mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 32.8
-
 (S)-malate mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 46.6
-
 (S)-malate mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 47.2
-
 (S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 61.1
-
 (S)-malate mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 62.6
-
 (S)-malate wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 64.8
-
 (S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 81.7
-
 (S)-malate mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 118.9
-
 (S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.1.1.40 122.2
-
 (S)-malate wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays