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Literature summary extracted from

  • Erb, T.; Evans, B.; Cho, K.; Warlick, B.; Sriram, J.; Wood, B.; Imker, H.; Sweedler, J.; Tabita, F.; Gerlt, J.
    A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis (2012), Nat. Chem. Biol., 8, 926-932 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum
-
 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum
-
 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum S1
-
 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum S1
-
 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum NCIMB 8255
-
 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum NCIMB 8255
-
 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum ATH 1.1.1
-
 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum ATH 1.1.1
-
 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum ATCC 11170
-
 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum ATCC 11170
-
 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum LMG 4362
-
 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum LMG 4362
-
 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum DSM 467
-
 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate Rhodospirillum rubrum DSM 467
-
 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.6 Rhodospirillum rubrum Q2RSU5
-
-
2.8.4.6 Rhodospirillum rubrum DSM 467 Q2RSU5
-
-
5.3.3.23 Rhodospirillum rubrum Q2RSU7
-
-
5.3.3.23 Rhodospirillum rubrum ATCC 11170 Q2RSU7
-
-
5.3.3.23 Rhodospirillum rubrum ATH 1.1.1 Q2RSU7
-
-
5.3.3.23 Rhodospirillum rubrum DSM 467 Q2RSU7
-
-
5.3.3.23 Rhodospirillum rubrum LMG 4362 Q2RSU7
-
-
5.3.3.23 Rhodospirillum rubrum NCIMB 8255 Q2RSU7
-
-
5.3.3.23 Rhodospirillum rubrum S1 Q2RSU7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + L-dithiothreitol
-
 Rhodospirillum rubrum 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dithiothreitol
-
 ?
2.8.4.6 S-methyl-1-thio-D-xylulose 5-phosphate + L-dithiothreitol
-
 Rhodospirillum rubrum DSM 467 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)dithiothreitol
-
 ?
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum S1 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum NCIMB 8255 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum ATH 1.1.1 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum ATCC 11170 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum LMG 4362 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum DSM 467 S-methyl-1-thio-D-ribulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum S1 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum NCIMB 8255 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum ATH 1.1.1 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum ATCC 11170 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum LMG 4362 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r
5.3.3.23 S-methyl-5-thio-D-ribulose 1-phosphate
-
 Rhodospirillum rubrum DSM 467 S-methyl-1-thio-D-xylulose 5-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.8.4.6 MTXu 5-P methylsulfurylase
-
 Rhodospirillum rubrum
2.8.4.6 Rru_A2000
-
 Rhodospirillum rubrum
5.3.3.23 ribulose-1,5-bisphosphate carboxylase/oxygenase-like protein
-
 Rhodospirillum rubrum
5.3.3.23 RLP
-
 Rhodospirillum rubrum
5.3.3.23 rlp Rru_A1998 locus name Rhodospirillum rubrum
5.3.3.23 RuBisCO-like protein
-
 Rhodospirillum rubrum

General Information

EC Number General Information Comment Organism
2.8.4.6 physiological function a gene disruption strain accumulates a mixture of MTRu-5P and MTXu-5P. Existence of a link in bacterial central carbon metabolism between S-adenosylmethionine-dependent polyamine metabolism and isoprenoid biosynthesis. 5'-methylthioadenosine, which is derived from polyamine biosynthesis, is first transformed into 5-methylthio-D-ribose-1-phosphate. 5-methylthio-D-ribose-1-phosphate is further isomerized to 5-methylthio-D-ribulose-1-phosphate, which then serves as substrate for the RubisCO-like protein. Owing to the combined action of the RubisCO-like protein and the cupin protein, methanethiol is released, and the phosphorylated ketose coproduct is converted into 1-deoxy-D-xylulose 5-phosphate and further channeled into isoprenoid biosynthesis Rhodospirillum rubrum
5.3.3.23 evolution in contrast to true D-ribulose-1,5-bisphosphate carboxylase/oxygenases (RubisCOs), ribulose-1,5-bisphosphate carboxylase/oxygenase-like protein s (RLPs) lack essential, conserved active site residues. Consequently, none of these proteins tested so far has been shown to catalyze the carboxylation of D-ribulose-1,5-bisphosphate. Based on phylogenetic analysis, active site differences and genome context, at least six different RLP-subfamilies are identified that are assumed to use different substrates and catalyze different reactions Rhodospirillum rubrum
5.3.3.23 malfunction disruption of the RLP gene results in a 5'-methylthioadenosine (MTA)-deficient growth phenotype under aerobic conditions Rhodospirillum rubrum
5.3.3.23 metabolism the Rhodospirillum rubrum genome lacks most of the canonical methionine salvage pathway genes and, therefore, might use an alternative strategy to utilize 5'-methylthioadenosine (MTA) aerobically as its sole sulfur source. Rhodospirillum rubrum RLP is involved in another central metabolic pathway, in which the polyamine biosynthesis dead-end product MTA is channelled into isoprenoid metabolism with concomitant release of the volatile gas methanethiol. The proposed MTA-isoprenoid pathway fits to the organism's physiology, and phylogenetic analysis indicate that a number of organisms make use of this metabolic shunt. Overview Rhodospirillum rubrum
5.3.3.23 physiological function the Rhodospirillum rubrum RLP enzyme functions in 5'-methylthioadenosine (MTA) metabolism. Wild type Rhodospirillum rubrum can grow with MTA as sole source of sulfur. When incubated in vitro with 5-methylthio-D-ribulose-1-phosphate (MTRu-1P), an intermediate of the canonical methionine salvage pathway, recombinant Rhodospirillum rubrum RLP catalyzes a 1,3-isomerization reaction (presumably two successive 1,2-isomerization reactions) to yield a 1:3 mixture of 1-methylthio-ribulose-5-phosphate (MTRu-5P) and 1-methylthio-xylulose-5-phosphate (MTXu-5P) Rhodospirillum rubrum